1Parul Institute of Pharmacy, Parul University, Vadodara-391760, Gujarat, India
*Corresponding Author: Bera Kinjal, Parul Institute of Pharmacy, Parul University, Vadodara-391760, Gujarat, India. Email: kinjal.bera@paruluniversity.ac.in
Received: 16th Dec, 2025; Revised: 8th Feb 2026; Accepted: 12th Feb, 2026; Available Online: 28th Feb, 2026
Oxidative stress mediated hepatocellular damage and inflammation are major key factors responsible for the occurrence of liver diseases. The plants possessing antioxidant and anti inflammatory potential are significantly explored as safer alternatives for hepatoprotection. Ficus amplissima has been traditionally used in the management of hepatic disorders like jaundice. This study aimed to evaluate the hepatoprotective potential of the ethanolic extract of Ficus amplissima leaf (EEFL) against carbon tetrachloride (CCl₄) induced liver injury. The prepared EEFL was standardized based on total flavonoid content, supported by chromatographic profiling and quantitate estimation of ursolic acid. Antioxidant activity was evaluated using the DPPH assay. Hepatotoxicity was induced by CCl₄ administration in Wistar rats, which were randomly divided into five groups (n = 6): normal control, CCl₄ treated, silymarin treated (100 mg/kg), and EEFL treated groups (100 and 250 mg/kg, orally). Serum biochemical markers of liver function include alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), Hepatic antioxidant enzymes like glutathione (GSH) and catalase (CAT), inflammatory markers (TNF-α and IL6), total bilirubin, total protein, albumin and lipid profile parameters were quantified. Histopathological changes were examine using hematoxylin and eosin staining. Standardization of the EEFL revealed high flavonoid content (59.57 ± 1.49%). The EEFL exhibited notable antioxidant potential with an IC₅₀ value of 60.20 µg/mL. Treatment with EEFL (250 mg/kg) significantly ameliorated the biochemical variations (p < 0.05–0.001) by restoring liver enzyme levels, and lipid profile. The EEFL produced a significant reduction in TNF-α and IL-6 level compared with the disease control group (****p <0.0001). Histopathological findings suggest reduced inflammation, and reduced cellular infiltration in EEFL treated groups. These findings provide experimental validation for the use of Ficus amplissima as hepatoprotective agent.
Keywords: Ficus amplissima; hepatoprotection; carbon tetrachloride; oxidative stress; antioxidant enzymes; IL6, TNF-α, liver injury.
How to cite this article: Bera K*, Choudhary V, Patel K, Patel M, Bhatt H, Parmar A, Protective Effect of Ficus Amplissima Leaf Extract on CCL₄ Induced Hepatotoxicity via Modulation of Oxidative Stress and Inflammatory Markers. Int J Drug Deliv Technol. 2026; 16(2): 511-520; DOI: 10.25258/ijddt.16.2.57
Source of support: Nil.
Conflict of interest: None