1Pravara Rural College of Pharmacy, Pravaranagar, Loni (Bk), Ahmednagar, Maharashtra, India, 413736
Corresponding Author: Santosh B. Dighe, Pravara Rural College of Pharmacy, Pravaranagar, Loni (Bk), Ahmednagar, Maharashtra, India, 413736. Email: sbdigheprcop@gmail.com, santosh.dighe@pravara.in
Received: 16th Dec, 2025; Revised: 8th Feb 2026; Accepted: 12th Feb, 2026; Available Online: 28th Feb, 2026
Objectives: The present study aimed to develop and evaluate a polyherbal formulation (PHF) comprising Bacopa monnieri and Vitis vinifera for its neuroprotective and nootropic activity in a scopolamine-induced amnesia model in mice.
Methods: Plant extracts were standardized and incorporated into a suspension formulation optimized using a 3-level, 2-factor full factorial design. The optimized batch was selected based on key evaluation parameters including pH, sedimentation volume, and redispersibility. Neuroprotective efficacy was assessed using Y-maze and Elevated Plus Maze (EPM) behavioral models, followed by biochemical assays (AChE, MDA, GSH) and histopathological analysis.
Results: Scopolamine significantly impaired memory as shown by reduced % alternation (18.43 ± 1.32) in the Y-maze and increased transfer latency (91.39 ± 0.941 s) in the EPM. Treatment with PHF at 400 mg/kg significantly improved % alternation (63.73 ± 1.86) and reduced latency (26.24 ± 0.60 s). Biochemically, PHF reduced AChE activity (0.720 ± 0.019 ng/ml), decreased MDA (16.86 ± 0.22 nmol/g), and restored GSH (2675 ± 36.60 µg/g). Histopathology confirmed preservation of hippocampal structure in PHF-treated animals. The optimized batch was selected based on superior neuroprotective efficacy at 400 mg/kg.
Conclusion: The optimized PHF exhibited potent nootropic and neuroprotective effects through dual mechanisms involving cholinergic regulation and antioxidant activity. These findings suggest that the formulation holds promise for future clinical translation as an adjunct therapy for neurodegenerative disorders such as Alzheimer's disease.
Keywords: Polyherbal formulation, Scopolamine, Nootropic activity, AChE, Oxidative stress, Behavioral models.
How to cite this article: Dighe SB, Kadale PS, Tambe MS, Mandlik TV, Bakale SS, Neuroprotective and Nootropic Assesment of Polyherbal Formulation Against Scopolamine Induced Cognitive Impairment. Int J Drug Deliv Technol. 2026; 16(2): 521-533; DOI: 10.25258/ijddt.16.2.58
Source of support: Nil.
Conflict of interest: None