aDepartment of Pharmaceutical Sciences and Technology, Maharaja Ranjit Singh Punjab Technical University, Bathinda, 151001, Punjab, India.
Quality control processes influence the cost of pharmaceutical product manufacturing; hence, a simple, economical, and validated analytical method is required. Thus, this research aimed to develop a sensitive, precise and cost-effective ultraviolet spectrophotometric method for quantification of donepezil hydrochloride in bulk and within biological samples.
The method used methanol as a solvent and was validated for linearity, robustness, accuracy, precision, limit of detection/quantification. The results showed a regression coefficient value (approx. 0.999) at 271 nm. The method showed high sensitivity with a limit of detection (1.031 µg/mL) and quantification (3.123 µg/mL). The method indicated excellent accuracy and precision with a relative standard deviation (< 1%). The in-vivo samples showed significant enhancement of drug levels in the brain with intranasal administration as compared to the oral route within 2 hours.
The developed method was found to be simple, robust and accurate for routine analysis in bulk, pharmaceutical formulations and biological samples.
Keywords: Ultraviolet spectrophotometry; Donepezil Hydrochloride, Biological Samples; Nanoparticles; Intranasal.
How to cite this article: Garg Y, Bhatia A, Development And Validation Of A New Ultraviolet Spectrophotometric Method For Quantification Of Donepezil In Nanoparticles And Biological Samples. Int J Drug Deliv Technol. 2026;16(2): 66-72. DOI: 10.25258/ijddt.16.2.08
Source of support: Nil.
Conflict of interest: None