1Research Scholar, School of Pharmacy and Research, Dev Bhoomi Uttarakhand University, Dehradun, Uttarakhand, India 248007
2*School of Pharmacy and Research, Dev Bhoomi Uttarakhand University, Dehradun, Uttarakhand, India 248007
3College of Pharmacy, Shivalik Campus, Dehradun, Uttarakhand, India 248197
Background: Urolithiasis is a common kidney condition characterized by the formation of stones, mainly formation of calcium oxalate crystals. High recurrence rate and possible side effects are linked to current strategies. The safety profile and synergistic pharmacological effects of polyherbal preparations have attracted increasing attention. The aim of the current study was to assess the antiurolithic potential of a polyherbal formulation made from three medicinal plants in rats experimentally induced to develop urolithiasis.
Material & Method: Male Sprague Dawley rats were given drinking water containing 0.75% ethylene glycol and 1% ammonium chloride for 28 days in order to establish experimental urolithiasis. The animals were divided into four groups (I, II, III, IV): standard drug-treated, disease-control, polyherbal-treated, and normal-control. During treatment, the formulation was administered orally. Urine parameters, including oxalate, phosphate, and calcium, were examined, as were biochemical parameters like serum creatinine, urea, uric acid, and calcium. To evaluate renal damage, including crystal deposition and renal damage, the kidney tissue was examined histopathologically. One-way ANOVA and post-hoc test were used for statistical analysis.
Result: Serum creatinine, urea, and uric acid, and urinary oxalate level were markedly increased in the disease control group after ethylene glycol and ammonium chloride were administered. These biochemical markers were significant (P < 0.05) return to normal after treatment with the polyherbal formulation. Additionally, the formulation enhanced the histologic architecture of the kidneys and decreased calcium oxalate crystal deposition.
Conclusion: The polyherbal formulation demonstrated strong anti-urolithic activity by restoring metabolic homeostasis and inhibiting the formation of renal crystals. These results point to its potential to aid in the natural treatment of urolithiasis.
Keywords: Antiurolithic activity, Polyherbal Formulation, Ethylene glycol-induced urolithiasis, Calcium oxalate crystallization.
How to cite this article: Kumar B, Parashar T, Mukhopadhyay S. Anti-Urolithic Activity of Polyherbal Formulation against Ethylene Glycol-induced Urolithiasis in Sprague Dawley Rats. Int J Drug Deliv Technol. 2026;16(2): 776-784. DOI: 10.25258/ijddt.16.1.83
Source of support: Nil.
Conflict of interest: None