International Journal of Drug Delivery Technology
Volume 16, Issue 2, 2026

Extraction, Qualitative and Quantitative analysis of Bauhinia variegata and Grevillea robusta

Yogesh Tiwari1, Tarun Parashar2*, Sayantan Mukhopadhyay3

1Research Scholar, School of Pharmacy and Research, Dev Bhoomi Uttarakhand University, Dehradun, Uttarakhand, India 248007

2*School of Pharmacy and Research, Dev Bhoomi Uttarakhand University, Dehradun, Uttarakhand, India 248007

3College of Pharmacy, Shivalik Campus, Dehradun, Uttarakhand, India 248197

Received: 15th Dec, 2025; Revised: 10th Feb 2026; Accepted: 12th Feb, 2026; Available Online: 28th Feb, 2026

ABSTRACT

Background: B. variegata is an indigenous species of China, Asia, and the tropical areas of the world. It belongs to the Leguminosae family. G. Robusta is a native of Eastern Australia, commonly known as the Australian silver oak. It is used to treat wounds, tumors, hemorrhoids, diarrhea, constipation, piles, edema, leprosy, and shows strong antioxidant effects. The main objective of this study was to perform leaf extraction of B. variegata and G. robusta and to perform phytochemical screening of the active constituents in the extracts.

Materials and Methods: The hydroalcoholic, ethanolic extraction of B. variegata and G. robusta leaves was performed using a Soxhlet apparatus. Active constituents in the extract were identified using phytochemical screening (qualitative and quantitative). Characterization of these extracts was carried out using UV spectroscopy and FTIR spectra.

Results: The % yield of B. variegata ethanolic-based extract was found to be 1.22% w/w, and hydroalcoholic-based extract 0.27% w/w. G. robusta percent yield 0.67% w/w and 0.27% w/w, respectively. B. variegata has a larger quantity of bioactive compounds, such as TPC 85.4 mg/g and TFC 64.2 mg/g. G. robusta have greater amount of tannin, 32.4 mg/g. FTIR peaks show O-H, C-H, C-O, and C=O.

Discussion: The biological activities of the active components found in B. variegata and G. robusta leaves must be identified.

Major Findings: This study showed that both plants produced bioactive compounds when extracted effectively. The secondary metabolites, including alkaloids, saponins, flavonoids, and terpenoids, were confirmed by phytochemical screening. Spectroscopy revealed functional groups consistent with pharmacologically significant phytochemicals.

Keywords: B. variegata, G. robusta, Phytochemical Screening, TPC, TFC, and FTIR.

Graphical Abstract: Presented in the original article.

How to cite this article: Tiwari Y, Parashar T, Mukhopadhyay S. Extraction, Qualitative and Quantitative analysis of Bauhinia variegata and Grevillea robusta. Int J Drug Deliv Technol. 2026;16(2): 785-791. DOI: 10.25258/ijddt.16.1.84

Source of support: Nil.

Conflict of interest: None