A simple, accurate, and robust RP-HPLC method was developed and validated for the quantitative estimation of Nebivolol Hydrochloride in tablet dosage form. The chromatographic conditions were optimized using a C18 column and a suitable mobile phase to obtain sharp and symmetrical peaks with acceptable retention time. The preliminary analytical performance of the developed method was assessed through system suitability studies, and further validation parameters such as linearity, accuracy, precision, LOD, LOQ, specificity, and robustness were evaluated as per ICH guidelines. The method showed excellent linearity within the selected concentration range and demonstrated high precision with %RSD values within limits. Accuracy results confirmed acceptable recovery, indicating that the method is reliable for estimation. Overall, the validated RP-HPLC method exhibited strong suitability for routine quality-control analysis of Nebivolol HCl formulations.
Keywords: Nebivolol Hydrochloride, RP-HPLC, method development, validation, ICH guidelines.
How to cite this article: Kumari V, Nayak G, Namdeo P, Analytical Method Development And Validation Of Nebivolol HCl In Tablet Dosage Form By RP-HPLC Method. Int J Drug Deliv Technol. 2026;16(2s): 559-567; DOI: 10.25258/ijddt.16.559-567