1Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Porur, Chennai, Tamil Nadu, India
Medicinal plants continue to be an important source of bioactive compounds with significant therapeutic potential. In this context, the present study focuses on the isolation and characterization of phytoconstituents from Myxopyrum serratulum A.W. Hill using standard extraction and chromatographic techniques. M. serratulum, a climbing shrub belonging to the family Oleaceae, is traditionally used in indigenous systems of medicine for the treatment of wounds, inflammation, scabies, infections, and metabolic disorders. In the present work, the dried and powdered aerial parts of the plant were subjected to extraction using 70% ethanol, followed by successive solvent extraction with solvents of increasing polarity. Preliminary phytochemical screening revealed the presence of flavonoids, alkaloids, phenolic compounds, terpenoids, and glycosides. Isolation of phytoconstituents was carried out using column chromatography, and the collected fractions were monitored by thin-layer chromatography (TLC). Iridoid glycosides were isolated using authenticated herbarium leaf material, while a pentacyclic triterpenoid, ursolic acid, was successfully isolated and purified through recrystallization. The isolated compounds were characterized and identified using sophisticated instrumental analytical techniques. High-performance thin-layer chromatography (HPTLC) fingerprinting was performed to determine the Rf values of the isolated compounds and to compare them with those of reference standards, confirming their identity and purity. The results of this study provide scientific support for the traditional medicinal uses of Myxopyrum serratulum and highlight its potential as a promising source of pharmacologically active phytoconstituents. Further studies on structural elucidation and biological evaluation of the isolated compounds may contribute to the development of novel therapeutic agents.
Keywords: Myxopyrum serratulum A.W. Hill; Phytochemical isolation; Column chromatography; Iridoid glycosides; HPTLC fingerprinting.
How to cite this article: Kumar M, Sheelarani T, Mani S. Phytochemical Isolation and Chromatographic Profiling of Bioactive Constituents from Myxopyrum Serratulum A.W. Hill. Int J Drug Deliv Technol. 2026;16(3): 173-181. DOI: 10.25258/ijddt.16.3.22
Source of support: Nil.
Conflict of interest: None