1Department of Biotechnology, B.M.S. College of Engineering, Bangalore-560019, Karnataka, INDIA
In Tissue Engineering, a Bio-scaffold restores the function or regenerates the tissue. Bio-based scaffolds offers an ideal matrix for proliferation of cells and deposition of extracellular matrix They ultimately contribute to the repair and regeneration of tissues in the specific regions of the biological system. This current work aims to make a chitosan scaffold coated with collagen extracted from fish waste, by the method of freeze drying and carry out eukaryotic (yeast) cell growth and mechanic studies and degradation assays on the scaffold. Characterization of scaffolds is done using bio analytical techniques FTIR, SEM and XRD. Estimation of protein content was done using Lowry's method and Bradford method. In addition, yeast cell growth on the scaffold is also studied. The total collagen extracted was 2.84 mg/10 gm of the bone fraction. Chitosan-collagen blend of 5% gave the highest swelling % when compared to other blends. 20% of the yeast cells were dies when grown on 4% blend of chitosan and collagen when compared to 4% viability loss with 5% scaffold. The physico-chemical and biological studies proved the chitosan-collagen scaffold to of significant importance in bio-medical applications.
Keywords: Chitosan, Collagen, Tissue Engineering, yeast culture, upcycling of fish waste, bio-scaffold.
How to cite this article: S P, MS C, Ashoka H, Biradar NG, S SS, N SN. Development of Sustainable Chitosan–Collagen Composite Scaffolds from Bio-Waste for Tissue Engineering Applications. Int J Drug Deliv Technol. 2026;16(3): 48-55. DOI: 10.25258/ijddt.16.3.7
Source of support: Nil.
Conflict of interest: None