Bryophytes are emerging as valuable sources of structurally diverse bioactive compounds with significant pharmacological Diabetes mellitus is a metabolic disease marked by blood glucose levels that are abnormally elevated. Pharmacological evaluation of polyherbal extract for in-vitro anti-oxidant and anti-diabetic potential. The fresh leaves of Azadirachta Indica, Ocimum Sanctum, Hibiscus rosa sinensis L., and fresh flowers of Clitoria ternatea L. were collected from the Prayagraj region, UP East, India. There were authenticated by the botanist at Botanical Survey of India, Allahabad, UP with the reference no. 2023-24/534. The dried leaves of Azadirachta indica, Ocimum sanctum and Hibiscus rosa sinensis L. were rendered into fine powder and extracted using hydroalcoholic (distilled water and ethanol; 1:1) solution through cold maceration process. The leaves of different plants were soaked in a beaker containing hydroalcoholic (distilled water and ethanol; 1:1) solution for 15 days with gradual stirrings, separately. Similarly, dried flowers of Clitoria ternatea L. was rendered into fine powders then soaked into a beaker containing hydroalcoholic (distilled water and ethanol; 1:1) solution for 15 days with gradual stirrings and thus extracted-out. Standardization of herbal extracts was performed. Isolation of phytochemicals was done through TLC and HPTLC. The antioxidant and anti-diabetic potentials were evaluated through DPPH scavenging assay, oxygen radical absorbance capacity assay and alpha amylase inhibitory assay. In results, the antioxidant and anti-diabetic potentials were found in decreasing order as DM-03> DM-02> DM-01> DM-04. Thus, among all the polyherbal formulations, DM-03 exhibited the highest pharmacological properties including 71.40% (DPPH antioxidant activity), at 200µg/ml, 3% (Oxygen Radical Absorbance Capacity Assay), at 1000µg/ml, 89.39±0.67% α-amylase inhibition. In conclusion, the antioxidant and antidiabetic response of the dried leaves of Azadirachta indica, Ocimum sanctum, flowers of Clitoria ternatea and leaves of Hibiscus rosa sinensis may be attributed to lower and sustain the cellular oxidation processes and its ability to enhance the responsiveness of tyrosine kinase to insulin.
Keywords: Azadirachta indica, Ocimum sanctum, Clitoria ternatea, Hibiscus rosa sinensis, Antioxidant, Anti-diabetic activity
How to cite this article: Singh S, Deva V., Pharmacological Evaluation of Polyherbal Extract for in-vitro anti-oxidant and anti-diabetic potential. Int J Drug Deliv Technol. 2026;16(3s): 242-252; DOI: 10.25258/ijddt.16.3s.32