Cortactin And SIRT1 Increased Expression Levels Are Associated With Meningioma Patients

Shubhangi Bhawangirkar ¹, Manas Panigrahi ², Satish Rao Immaneni ³, Prakash Babu Phanithi ^4*

¹Neuroscience Laboratory, Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad - 500046, Telangana, India.
Email: 16ltph02@uohyd.ac.in

²Department of Neurosurgery, Krishna Institute of Medical Sciences (KIMS), Secunderabad - 500003, Telangana, India.
Email: manasp1966@gmail.com

³Department of Laboratory Medicine, Krishna Institute of Medical Sciences (KIMS), Secunderabad - 500003, Telangana, India.
Email: satishrao.i@kimshospitals.com

^4*Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad - 500046, Telangana, India.
Phone: 040-23134584; Fax: 040-23010120; Room No: F-23/F-71, Neuroscience Laboratory
Email: prakash@uohyd.ac.in

*Corresponding Author: Prakash Babu Phanithi, Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad - 500046, Telangana, India. Phone: 040-23134584; Fax: 040-23010120; Email: prakash@uohyd.ac.in

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ABSTRACT

Meningiomas are the most prevalent CNS neoplasms in adults and display various cytological and histological features associated with higher morbidity and mortality rates and diagnostic complexity. SIRT1 is the class III histone deacetylase (HDAC) that plays a role in the metabolism, aging, and carcinogenesis of organisms and regulates senescence and apoptosis in cells but its role in meningioma is unclear. This study aims to check the expression patterns and interactions of cortactin and SIRT1 proteins in different grades of meningiomas and control arachnoid tissue.

In the experimental groups of meningioma, human tissue samples from the three grades of meningioma (n=28) and control arachnoid tissues (n=12) were employed for assessing the expression of cortactin and SIRT1 proteins via immunoblotting, immunohistochemical staining, and immunofluorescence staining. Results revealed a significant increase in cortactin and SIRT1 expression in high-grade meningiomas compared to arachnoid tissue. Co-expression analysis with ki67 revealed correlations between cortactin/SIRT1 and proliferative activity, particularly in grade II and grade III meningiomas. Co-immunoprecipitation assays confirmed a direct interaction between cortactin and SIRT1, which intensified with tumor severity. Here, we report the in vivo interaction between cortactin and SIRT1, revealed by fluorescence resonance energy transfer (FRET) microscopy.

In conclusion, this study emphasizes the significance of cortactin and SIRT1 as biomarkers for diagnosis and prognosis in meningiomas, suggesting their potential as targets for therapy.

Keywords: Cortactin, SIRT1, invasion, proliferation, metastasis, meningioma.

How to cite this article: Bhawangirkar S, Panigrahi M, Immaneni SR, Phanithi PB. Cortactin and SIRT1 increased expression levels are associated with meningioma patients. Int J Drug Deliv Technol. 2026;16(3s): 817-831; DOI: 10.25258/ijddt.16.3s.99

Source of support: Nil.

Conflict of interest: None