International Journal of Drug Delivery Technology
Volume 16, Issue 4s

Development And Validation Of A UV–Visible Spectrophotometric Method Using Simultaneous Equation Analysis For Standardization Of Curcumin And Quercetin In Herbal Formulations

¹Landge Dhananjay Ashok, ²Pradyumn Tiwari, ³Prasanth S S*, ⁴Shainda Laeeq, ⁵Aparna Dwivedi, ⁶Shiv Kumar Gupta, ⁷Debasish Maity, ⁸Piyali Khamkat

¹Associate Professor, Adsul College of Pharmacy, Chas, Ahmednagar, Maharashtra. 414005
²Assistant Professor, School of Pharmacy, ITM University, Gwalior.
³Professor & HOD, MGM Silver Jubilee College of Pharmacy, Kilimannoor, Trivandrum.
⁴Associate Professor, Department of Pharmaceutical Chemistry, Maharana Pratap College of Pharmaceutical Sciences, Mandhana, Kanpur, Uttar Pradesh.
⁵Associate Professor, Department of pharmaceutics, Maharana Pratap College of Pharmaceutical Sciences, Kothi Mandhana, Kanpur, Uttar Pradesh.
⁶Professor, Saraswathi College of Pharmacy, NH 24, Anwarpur Pilkhuwa, Hapur, Uttar Pradesh.
⁷,⁸Department of Pharmaceutical Technology, Brainware University, 398, Ramkrishnapur Road, Barasat, Kolkata -125, West Bengal, India.
Corresponding Author:
³Prasanth S S*
³Professor & HOD, MGM Silver Jubilee College of Pharmacy, Kilimannoor, Trivandrum.

ABSTRACT

Background: Standardization of herbal formulations remains a major analytical challenge due to the presence of multiple bioactive phytoconstituents with overlapping spectral characteristics. Curcumin and quercetin are widely recognized marker compounds with established therapeutic relevance; however, routine quality control methods for their simultaneous estimation are often limited by cost, complexity, and accessibility.

Methods: UV–Visible spectrophotometric method based on simultaneous equation analysis was developed and validated for the concurrent quantification of curcumin and quercetin in herbal formulations. The method utilized methanol as solvent, with analytical wavelengths selected at 425 nm and 370 nm. Validation was performed in accordance with ICH Q2(R1) guidelines, assessing linearity, accuracy, precision, sensitivity, robustness, and applicability to commercial herbal products.

Results: The method demonstrated excellent linearity with correlation coefficients exceeding 0.999 for both analytes. Accuracy studies yielded recovery values within 98–102%, while precision analysis showed %RSD values below 2%. Low limits of detection and quantification confirmed adequate sensitivity. Assay results of marketed herbal formulations were within acceptable pharmacopeial limits, indicating suitability for routine quality control.

Conclusion: The validated method provided a rapid, economical, and reliable analytical tool for simultaneous standardization of curcumin and quercetin in herbal formulations, supporting improved quality assurance and regulatory compliance.

Keywords: Curcumin; Quercetin; UV–Visible spectrophotometry; Simultaneous equation method; Herbal standardization; Method validation

How to cite this article: Ashok LD, Tiwari P, S S P, Laeeq S, Dwivedi A, Gupta SK, Maity D, Khamkat P, Development And Validation Of A UV–Visible Spectrophotometric Method Using Simultaneous Equation Analysis For Standardization Of Curcumin And Quercetin In Herbal Formulations. Int J Drug Deliv Technol. 2026;16(4s): 93-101; DOI: 10.25258/ijddt.16.93-101