International Journal of Drug Delivery Technology
Volume 16, Issue 4s

Integrating In Silico and In Vitro Studies to develop phytobiotic therapeutics for Aeromonas hydrophila in Aquaculture

Vishali Krishnan1, Jamal Mohamed1*, Sunandha Jeeva Bharathi G2, Esther Angel Peter Ravi Kumar1, Ravi Mani3*, N.MD. Azmathullah1

1Unit of Aquatic Science, PG & Research Department of Zoology, The New College, Affiliated to University of Madras, Chennai, Tamil Nadu, India.
2Department of Microbiology, Alagappa University, Karaikudi, Tamil Nadu, India.
3*Centre for Ocean Research, Sathyabama Institute of Science and Technology, Sathyabama Ocean Research Field Facility, Chennai, Tamil Nadu, India.

Note: Corresponding author email addresses were not provided in the user's message.

ABSTRACT

Aeromonas hydrophila is a major bacterial pathogen in aquaculture, known for causing many fish diseases. The rise in antibiotic resistance genes due to antibiotic usage has led to restrictions on antibiotic use, making phytochemicals a promising alternative. In this study, the computational and in-vivo analysis focused on Hemolysin protein as a putative of the Diosgenin against Aeromonas hydrophila. In previous literature studies, 376 bioactive compounds were identified from 9 different plants with potent antibacterial activity. The virulence protein of A.hydrophila was identified as Hemolysin. Phytocompounds 3-D structures used from PubChem and Molecular docking were performed. Diosgenin showed a good binding affinity with the Hemolysin with a score of -8.7. The most potent inhibitor was carried down for Minimum Inhibitory Concentration (MIC) and Anti-hemolytic assay. MIC indicated Diosgenin has dose-dependent antibacterial activity, and also diosgenin showed potent antibacterial activity against Aeromonas hydrophila with the minimum 25 μM concentration, Meanwhile, diosgenin significantly inhibited the hemolytic activity of A.hydrophila. The study concludes that Diosgenin, a bioactive phytocompound, effectively inhibits the virulence and hemolytic activity of Aeromonas hydrophila, demonstrating its potential as a natural alternative to antibiotics for managing bacterial infections in aquaculture.

Keywords: Diosgenin, Molecular docking, Hemolysin, Bioactive compounds, Antibacterial Activity.

How to cite this article: Krishnan V, Mohamed J, Bharathi SJG, Kumar EAPR, Mani R, Azmathullah NM, Integrating In Silico and In Vitro Studies to develop phytobiotic therapeutics for Aeromonas hydrophila in Aquaculture. Int J Drug Deliv Technol. 2026;16(4s): 950-957; DOI: 10.25258/ijddt.16.4s.111

Source of support: Nil

Conflict of interest: None