The development of standardized herbal supplements requires integration of ethnopharmacological knowledge with experimental validation and quality control. The present investigation describes the formulation, phytochemical standardization, safety evaluation, and experimental validation of a novel polyherbal diuretic supplement, Azemkofit, prepared from locally sourced medicinal plants: Alhagi maurorum (camel thorn), Equisetum arvense (horsetail), Zea mays stigma (corn silk), Calendula officinalis (calendula), and Matricaria chamomilla (chamomile). Hydroethanolic extracts were prepared using optimized extraction conditions and standardized based on total phenolic and flavonoid content. Acute oral toxicity was evaluated in Wistar rats following internationally accepted guidelines. Diuretic activity was assessed using metabolic cage studies with furosemide (20 mg/kg) as reference control. Urinary output, electrolyte excretion (Na⁺, K⁺, Cl⁻), diuretic index, and saluretic index were calculated. The formulation produced statistically significant, dose-dependent increases in urine volume and sodium excretion without excessive potassium depletion (p < 0.05). Accelerated stability testing demonstrated phytochemical stability over 90 days. The findings support the potential of Azemkofit as a safe and standardized plant-based diuretic supplement suitable for further pharmacodynamic and clinical evaluation.
Keywords: Polyherbal formulation, pharmacognosy, diuretic activity, phytochemical standardization, herbal supplement, electrolyte balance.
How to cite this article: Javokhir A, Zaynab S, Dilfuza M, Nargiza M, Elmira S, Sitora A, Gulmira M. Obtaining a new diuretic biologically active supplement "Azemkofit" based on local medicinal plants. Int J Drug Deliv Technol. 2026;16(4s): 241-244; DOI: 10.25258/ijddt.16.4s.29
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Conflict of interest: None