International Journal of Drug Delivery Technology
Volume 16, Issue 5s, 2026

Preliminary Screening and Antioxidant Activity of Endophytic Fungi Isolated from Ampelocissus latifolia (Roxb.) Planch.

1 Meenakshi Baher, 1* Basanti Brar, 2 Ashok Kumar, 2 Dr. Ajit Kumar

1Research Scholar, School of Microbiology, Om Sterling Global University, Hisar, Haryana, India

1*Associate Professor, HOD, School of Applied Sciences, Om Sterling Global University, Hisar, Haryana, India

2Seminal Applied Sciences Pvt. Ltd., Jaipur, Rajasthan, India

2Director and Principal Scientific Officer, Seminal Applied Sciences Pvt. Ltd., Jaipur, Rajasthan, India

ABSTRACT

Ampelocissus latifolia (Roxb.) Planch., a medicinal climber of the family Vitaceae, is known for its traditional therapeutic uses and rich phytochemical profile, including phenolics and flavonoids. In this study, five morphologically distinct endophytic fungi (AL01-01: Penicillium sp., AL01-04: Aspergillus niger, AL02-01: Fusarium moniliforme, AL02-02: Alternaria spp., AL02-03: Acremonium strictum) were isolated from its leaves. Microscopic identification, phytochemical screening, and quantification assays were performed to evaluate the presence of secondary metabolites and antioxidant potential. Phytochemical analysis revealed the presence of saponins, phenols, tannins, flavonoids, terpenoids, and alkaloids, with AL02-01 and AL02-03 showing the richest profiles. Total phenolic content (TPC) and total flavonoid content (TFC) increased with extract concentration, with AL01 isolates exhibiting higher TPC and TFC than AL02 isolates. Total antioxidant capacity (TAC), DPPH radical scavenging activity, and FRAP reducing power assays demonstrated that AL02 isolates, particularly AL02-01, AL02-02, and AL02-03, possessed strong antioxidant potential, whereas AL01 isolates showed moderate activity. Overall, the study highlights that endophytic fungi from A. latifolia are promising sources of bioactive metabolites with significant antioxidant properties, supporting their potential for pharmaceutical and nutraceutical applications.

Keywords: Vitaceae, Therapeutic uses, Endophytic fungi, Antioxidant potential, Profiles, Phytochemicals, Traditional, Quantification, Leaves, Secondary metabolites.

How to cite this article: Baher M, Brar B, Kumar A, Kumar A. Preliminary Screening and Antioxidant Activity of Endophytic Fungi Isolated from Ampelocissus latifolia (Roxb.) Planch. Int J Drug Deliv Technol. 2026; 16(5s): 981-997; DOI: 10.25258/ijddt.16.5s.121

Source of support: Nil.

Conflict of interest: None