1Assistant Professor, Vidhyadeep College of Pharmacy, Surat, Vidhyadeep University, Surat, India
ORCID: https://orcid.org/0000-0002-4642-2087
Email: neetudharu148@gmail.com (Corresponding Author)
2Principal, Khyati College of Pharmacy, Palodia, Ahmedabad, Gujarat 382115, India
ORCID: https://orcid.org/0009-0008-0830-1875
Received: 16th Dec, 2025; Revised: 8th Feb, 2026; Accepted: 12th Feb, 2026; Available Online: 28th Feb, 2026
Edible oils are major dietary sources of essential fatty acids and natural antioxidants. The present study aimed to develop and validate a rapid and reliable HPLC method for simultaneous estimation of palmitic acid, oleic acid, linoleic acid, and tocopherol in selected branded edible oils available in the Indian market. The method was validated according to ICH Q2(R1) guidelines for linearity, precision, accuracy, robustness, limit of detection (LOD), and limit of quantification (LOQ). Heavy metal content was determined using inductively coupled plasma–optical emission spectroscopy (ICP-OES), and moisture content was analyzed using Karl Fischer titration.
The developed method showed excellent linearity (R² > 0.999), precision (%RSD < 2%), and recovery (98–102%). Oleic acid was found to be the predominant fatty acid (44.99%), followed by palmitic acid (32.82%), linoleic acid (10.33%), and tocopherol (8.32%). Heavy metal levels were within permissible limits, while moisture content in certain samples exceeded recommended values. The validated method is suitable for routine quality control analysis of edible oils.
Keywords: Edible oils, HPLC, Tocopherol, Fatty acids, ICP-OES, Karl Fischer, Method validation.
How to cite this article: Dharu NR, Patani PV. Qualitative and Quantitative Estimation of Important Chemical Constituents of Some Branded Edible Oils Available in Indian Market. Int J Drug Deliv Technol. 2026;16(5s): 617-626; DOI: 10.25258/ijddt.16.5s.79
Source of support: Nil.
Conflict of interest: None