1Associate Professor, Department of Pharmacology, Shriram College of Pharmacy, Banmore, Morena (M.P), India
Email: ravindra.mishra1412@gmail.com
2Scholar, M-Pharma, Department of Pharmacology, Shriram College of Pharmacy, Banmore, Morena (M.P), India
3Principal & Professor, Department of Pharmacognosy, Shriram College of Pharmacy, Banmore, Morena (M.P), India
Corresponding Author:
Ravindra Mishra
Associate Professor, Department of Pharmacology, Shriram College of Pharmacy, Banmore, Morena (M.P), India
Email: ravindra.mishra1412@gmail.com
Conflict of interest: All the authors have no conflict of interest
Hypertension remains a major global health burden, associated with cardiovascular morbidity and mortality. Despite effective synthetic therapies, limitations such as side effects and poor adherence highlight the need for alternative remedies. Euryale ferox Salisb. (fox nut/makhana) is traditionally used in Asian medicine as a cardiotonic; however, its antihypertensive effects have not been systematically validated. This study aimed to evaluate the in vivo antihypertensive potential of ethanolic seed extract of E. ferox in L-NAME-induced hypertensive rats. Seeds were collected, authenticated, and extracted using a Soxhlet apparatus. Hypertension was induced by oral administration of L-NAME (40 mg/kg/day, 4 weeks). Rats were divided into six groups: normal control, hypertensive control, standard (Captopril 10 mg/kg), and three test groups receiving E. ferox extract (100, 200, and 400 mg/kg). Hemodynamic parameters, biochemical markers (NO, renin, angiotensin II, lipid profile, renal markers, oxidative stress enzymes), and histopathology were evaluated. Extract-treated rats showed significant dose-dependent reductions in SBP and DBP (p < 0.05), with the 400 mg/kg dose showing effects comparable to captopril. Antioxidant markers (SOD, catalase, GSH) increased while MDA decreased, and renal/lipid profiles normalised significantly. Histological analysis revealed restoration of kidney, heart, and aortic architecture. Findings suggest that E. ferox exerts antihypertensive activity through nitric oxide restoration, renin–angiotensin system modulation, antioxidant action, and end-organ protection. These results support fox nut as a potential nutraceutical or adjunct therapy for hypertension.
Keywords: Euryale ferox, Hypertension, L-NAME model, Renin-angiotensin system, Oxidative stress, Phytotherapy.
How to cite this article: Mishra R, Rathore Y, Jain V. In Vivo Evaluation of the Antihypertensive Effect of Euryale ferox Salisb. Seed Extract in L-NAME-Induced Hypertensive Rats. Int J Drug Deliv Technol. 2026;16(6s): 976-985; DOI: 10.25258/ijddt.16.6s.127
Source of support: None
Conflict of interest: None