1,2School of Pharmacy, Devi Ahilya Vishwavidlaya, Indore, Madhya Pradesh
Arisaema tortuosum (Wall.) Schott is an ethnomedicinally significant plant lacking comprehensive phytochemical profiling. This study characterized tuber extracts prepared by sequential solvent extraction, with yields varying by polarity: chloroform (2.5%), ethyl acetate (2.9%), ethanol (6.3%), and aqueous (11.2%) w/w. Preliminary screening confirmed alkaloids, flavonoids, phenolics, and tannins. Ethanol extract showed superior total phenolic (0.878 mg Gallic Acid Equivalent/100 mg) and flavonoid (0.805 mg Quercetin Equivalent/100 mg). A validated HPTLC method was developed (ICH-compliant) which quantified lupeol with high linearity (R² = 0.999, LOQ 3.70 µg/spot), precision (intra-day %CV 0.67–1.03), accuracy (99.92–100.05% recovery), and sensitivity (LOD 1.22 µg/spot) content. Lupeol (Rf 0.67) was highest in ethanol extract (0.667% w/w), confirmed by HPTLC profiling and further characterized by GC-MS/MS analysis by matching retention times (sample: 31.85 min, standard: 31.86 min) and characteristic molecular ion (m/z 426). Cytotoxicity against A431 human epidermoid carcinoma cells revealed potent activity for the ethanol extract (IC₅₀ 13.49 µg/mL), approximately 12.5-fold stronger than lupeol standard (IC₅₀ 168 µg/mL). The ethanol extract of A. tortuosum is a promising anticancer lead, supported by a robust phytochemical profile and a validated HPTLC method for quality control.
Keywords: GC-MS/MS, HPTLC, ICH guideline, Method Development and Validation, NRU assay, A431 Cell Line.
How to cite this article: Malviya S, Dahima R, HPTLC Method Validation, GC-MS/MS Characterization and Anticancer Evaluation of Arisaema tortuosum Tubers against A431 Cells. Int J Drug Deliv Technol. 2026;16(6s): 266-281; DOI: 10.25258/ijddt.16.6s.32
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Conflict of interest: None