1PhD Research Scholar, Faculty of Pharmaceutical Sciences, Baba Mastnath University, Asthal Bohar, Rohtak, Haryana, India
2Assistant Professor, Faculty of Pharmaceutical Sciences, Baba Mastnath University, Asthal Bohar, Rohtak, Haryana, India
3Dean & Professor, Department of Pharmaceutical Sciences and Research, SGT University, Gurugram, Haryana, India
4Assistant Professor, University Institute of Pharmaceutical Sciences and Research, Chandigarh University, Punjab, India
5Lecturer, Uchha Madhyamik Vidyalya, Ghurghat, Siswan, Siwan, Bihar, India
6Research Scholar, Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, India
Corresponding Author:
Dr. Vikram Kumar
Faculty of Pharmaceutical Sciences, Baba Mastnath University, Asthal Bohar, Rohtak, Haryana, India
Email: rajivkarira@gmail.com
Background: Helicobacter pylori infection is a major etiological factor in the development of gastric ulcers and other gastric disorders. The need for safe and effective gastroretentive delivery systems has led to increasing interest in herbal formulations; however, their gastric safety must be thoroughly evaluated before further development.
Objective: The present study aimed to evaluate the in vitro cytotoxicity and gastric epithelial safety of anti-Helicobacter pylori herbal microspheres using the human gastric adenocarcinoma (AGS) cell line.
Methods: AGS cells were cultured under standard conditions and treated with different concentrations of the herbal microsphere formulation. Cell viability was assessed using the MTT assay, and the percentage of viable cells was calculated relative to untreated controls. Morphological evaluation of treated and control cells was performed using an inverted light microscope to observe changes in cellular integrity, attachment, and overall morphology.
Results: The MTT assay demonstrated high cell viability across all tested concentrations, indicating minimal cytotoxic effects of the formulation. No significant reduction in metabolic activity was observed when compared with control cells. Morphological examination further confirmed the preservation of normal epithelial characteristics, with treated cells exhibiting intact cell structure, proper attachment, and uniform distribution. The IC₅₀ value was found to be significantly higher than the effective concentration range, supporting the biocompatible nature of the microspheres.
Conclusion: The anti-Helicobacter pylori herbal microspheres exhibited excellent compatibility with AGS gastric epithelial cells, confirming their in vitro gastric safety. These findings suggest that the formulation holds strong potential for further development as a gastroretentive therapeutic system for gastric ulcer management.
Keywords: Helicobacter pylori; Herbal microspheres; AGS cell line; In vitro cytotoxicity; Gastric safety; Gastroretentive drug delivery; Gastric ulcer.
How to cite this article: Yadav R, Shukla Y, Chaturvedi S, Arun, Singh A, Ananya. Antiulcer Activity of Developed Herbal Floating Microspheres of Mangifera indica using Human Gastric Adenocarcinoma (AGS) Cell Lines. Int J Drug Deliv Technol. 2026;16(6s): 676-681; DOI: 10.25258/ijddt.16.6s.93
Source of support: None
Conflict of interest: None