*Corresponding Author: Dr. Sarita Bhandari, Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600077, Tamil Nadu, India. Email: saritabhandari1@gmail.com
Aim: This study aims to analyze the bio efficacy of calcium hydroxide incorporated silver nanoparticles synthesized from Acacia nilotica as intracanal medicaments.
Background: The primary objective of any endodontic treatment is the removal of the infectious tissue from the root canal which also involves the removal of debris formed during the process of removal and to prevent the reinfection. This majorly involves killing of microbes, the source of infection. This study utilizes the properties of calcium hydroxide incorporated silver nanoparticles synthesized from Acacia nilotica as intracanal medicament in endodontic treatment for the complete removal of microbes and prevention of recurrences.
Materials and Method: 1% Acacia nilotica extract was prepared and kept in stirring condition for complete dissolution. Filtration was done using Whatman No:1 filter paper and stored at 4°C. 1 milli molar of silver nitrate was prepared and added to the filtered extract drop by drop; color change was observed and the time period noted. Centrifugation was done at 6000 rpm for 10 mins at room temperature at 4°C. Crystal violet assay was performed. A 96-well plate was used; 100 μL of BHI medium was poured into 7 wells. 1% Ca(OH)₂ in serial dilution was poured into 6 wells. 50 μL of Enterococcus faecalis of OD600 = 0.1 was incubated at 37°C for 48 hours. Phosphate buffer saline (PBS) wash was done. 100 μL of 0.2% crystal violet was added (irrespective of control) and incubated for 20 to 30 mins. PBS wash was repeated to remove excess and unbound stain. 125 μL of 30% glacial acetic acid was mixed in all wells and an ELISA plate reader was used to measure the absorbance at 570 nm.
Results: AgNPs synthesized from Acacia nilotica incorporated with Ca(OH)₂ showed a high level of biofilm inhibition against the bacteria, of about more than 50% at a concentration of 0.25%, whereas the normal Ca(OH)₂ showed more than 50% biofilm inhibition at 1% and showed only 40.86% inhibition at 0.25% concentration.
Conclusion: The study showed that silver nanoparticles synthesized from Acacia nilotica incorporated with Ca(OH)₂ had better action as an intracanal medicament than calcium hydroxide alone.
Keywords: Acacia nilotica, Calcium Hydroxide, Enterococcus faecalis, Metal Nanoparticles, Enzyme-Linked Immunosorbent Assay.
How to cite this article: Britina G, Bhandari S, Venkataiah VS. Assessment of antibacterial properties of green synthesized silver nanoparticles derived from Acacia nilotica. Int J Drug Deliv Technol. 2026;16(7s): 343-350; DOI: 10.25258/ijddt.16.7s.36
Source of support: Nil.
Conflict of interest: None