International Journal of Drug Delivery Technology
Volume 16, Issue 7s

Running title: Muṣṭādi Siddhajala as Adjuvant to ORS

Exploring Muṣṭādi Siddhajala as a Novel Electrolyte Carrier in ORS and its adjuvant effect in Diarrhoea-Induced Dehydration: An Experimental Study in Wistar Rats

Mounika Buduru1*, Kirti Bhati2, Jayshree Dawane3, Sudeep Menon4, Dhananjay Patil5, Anuja Patil6

1*MD Scholar, Department of Swasthavritta and Yoga, College of Ayurved & Hospital, Bharati Vidyapeeth (Deemed to be University), Pune, Maharashtra

ORCID: https://orcid.org/0000-0003-4715-5846
2Professor, Department of Public Health and Nutrition, Maharashtra University of Health Sciences (Nasik), Regional Centre Pune, Maharashtra
3Professor, Department of Pharmacology, Medical College, Bharati Vidyapeeth (Deemed to be University), Pune, Maharashtra
4Professor and HOD, Department of Swasthavritta and Yoga, College of Ayurved & Hospital, Bharati Vidyapeeth (Deemed to be University), Pune, Maharashtra
5Associate Professor, Department of Swasthavritta and Yoga, College of Ayurved & Hospital, Bharati Vidyapeeth (Deemed to be University), Pune, Maharashtra
6Assistant Professor, Department of Swasthavritta and Yoga, College of Ayurved & Hospital, Bharati Vidyapeeth (Deemed to be University), Pune, Maharashtra

Reporting guidelines followed: ARRIVE 2.0

Trial registration: BVDUMC/6176/2024/01/05

Funding: This study is funded by the Central Council for Research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Government of India, under Scheme for Training in Ayurveda Research (PG-STAR) Session 2 for the PG Admission Batch 2022-23.

Study Site: Bharati Vidyapeeth Poona College of Pharmacy, Pune, Maharashtra, 411038

ABSTRACT

Background: Diarrhoea remains a major global health concern, primarily due to dehydration resulting from excessive loss of fluids and electrolytes. Although Oral Rehydration Solution (ORS) effectively corrects dehydration through sodium–glucose co-transport mechanisms, it does not significantly reduce stool frequency or intestinal secretion. Ayurveda describes Atīsāra as a disorder of impaired digestive function and doṣha imbalance, and classical formulations such as Muṣṭādi Siddhajala (MSJ) are indicated in its management.

Objective: To evaluate the adjuvant effect of Muṣṭādi Siddhajala combined with ORS in castor oil–induced diarrhoea and dehydration in Wistar rats.

Methods: Thirty healthy albino Wistar rats (150–250 g) were randomly allocated into five groups (n=6): Normal Control, Disease Control, ORS, MSJ, and MSJ+ORS. Diarrhoea was induced using castor oil (2 ml, orally). Interventions were administered in place of drinking water following induction. Outcome measures included diarrhoea severity score, dehydration score, body weight changes, urine output, and serum electrolyte levels (Na⁺, K⁺, Cl⁻). Statistical analysis was performed using Wilcoxon matched-pairs and Kruskal–Wallis tests, with p < 0.05 considered significant.

Results: The MSJ+ORS group demonstrated a significant reduction in diarrhoeal severity and improved hydration parameters compared to the Disease Control group (p < 0.05). Serum sodium and chloride levels were better preserved in the combination group, with reduced weight loss and improved urine output.

Conclusion: Muṣṭādi Siddhajala, when used as an adjuvant to ORS, demonstrated synergistic benefits in managing diarrhoea-induced dehydration, supporting its potential role in integrative therapeutic approaches.

Key words: Atīsāra, Diarrhoea, Dehydration, Oral Rehydration Solution, Muṣṭādi Siddhajala

How to cite this article: Buduru M, Bhati K, Dawane J, Menon S, Patil D, Patil A, Exploring Muṣṭādi Siddhajala as a Novel Electrolyte Carrier in ORS and its adjuvant effect in Diarrhoea-Induced Dehydration: An Experimental Study in Wistar Rats. Int J Drug Deliv Technol. 2026;16(7s): 20-25; DOI: 10.25258/ijddt.16.7s.4

Source of support: Nil

Conflict of interest: None