1Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai 600077, Tamil Nadu, India
*Corresponding author: saritabhandari1@gmail.com
Aim: This study aims to analyze the Bio efficacy of Calcium hydroxide incorporated silver nanoparticles synthesized from Acacia nilotica as intracanal medicaments.
Background: The primary objective of any endodontic treatment is the removal of the infectious tissue from the root canal which also involves the removal of debris formed during the process of removal and to prevent the reinfection. This majorly involves killing if microbes, the source of infection. This study utilizes the properties of Calcium hydroxide incorporated silver nanoparticles synthesized from Acacia nilotica as intracanal medicament in endodontic treatment for the complete removal of microbes and prevention of recurrences.
Materials and method: 1% Acacia nilotica is made and kept in stirring condition for complete dissolution. Filtration was done using whatman no:1 filter paper and stored at 4°C.1 milli molar of silver nitrate was prepared and added to the filtered extract drop by drop under color change observed and the time period is noted. Centrifuge was done at 6000 rpm for 10 mins at room temperature at 4°C.Crystal violet assay was done.96 well plate was taken.100 ml of BHI medium was poured in 7 wells .1% CaOH in serial dilution was poured upto 6 wells.50 ml of enterococcus faecalis of OD600=0.1 incubated at 37 °C for 48 hours. Phosphate buffer saline (PBS) wash was done.100μL of 0.2% of crystal violet irrespective of control was added and incubated for 20 to 30 mins. Again PBS wash was done to remove excess and unbound stains.125 μL of 30% glacial acetic acid is mixed in all wells and ELISA plate reader was used to measure the observents at 570 nm.
Results: AgNps synthesized from Acacia nilotica incorporated Ca(OH)₂ has a high level of biofilm inhibition against the bacteria of about more than 50% at a concentration of 0.25% whereas the normal Ca(OH)₂ showed more than 50% biofilm inhibition at 1% and showed only inhibition of 40.86% at 0.25% concentration.
Conclusion: the study showed silver nanoparticles synthesized from Acacia nilotica incorporated Ca(OH)₂ had better action as intracanal medicament than Calcium hydroxide.
Keywords: Acacia, Calcium Hydroxide, Enterococcus faecalis, Metal Nanoparticles, Enzyme-Linked Immunosorbent Assay.
How to cite this article: Britina G, Bhandari S, Venkataiah VS. Assessment of antibacterial properties of green synthesized silver nanoparticles derived from Acacia nilotica. Int J Drug Deliv Technol. 2026;16(7s): 564-571; DOI: 10.25258/ijddt.16.7s.59
Source of support: None
Conflict of interest: None