1Maratha Vidya Prasarak Samaj's College of Pharmacy, Nashik, Maharashtra, India
2Government College of Pharmacy, Amravati, Maharashtra, India
Received: ; Revised: ; Accepted: ; Available Online:
The present study designed to assess the antityrosinase and antioxidant activities of succeeding solvent extracts of Pleurotus ostreatus (oyster mushroom). Dried mushroom powder was successively extracted using hexane (HEPO), dichloromethane (DMEPO), ethyl acetate (EAEPO), and ethanol (EEPO). Physicochemical parameters and preliminary phytochemical screening were completed using standard pharmacognostic methods. Antioxidant activity was assessed by DPPH, hydrogen peroxide (H₂O₂), and ABTS radical scavenging assays, while tyrosinase inhibitory activity was assessed spectrophotometrically using L-tyrosine as substrate. Hydroquinone monomethyl ether (HQMME) and arbutin were used as reference standards. Amongst all extracts, EAEPO and EEPO exhibited superior antioxidant activity. In the DPPH assay, IC₅₀ values for HEPO, DMEPO, EAEPO, and EEPO were 96.86, 113.65, 63.62, and 58.69 μg/mL, respectively, compared to 81.58 μg/mL for ascorbic acid. In the H₂O₂ scavenging assay, EEPO showed strong activity with an IC₅₀ of 113.11 μg/mL, comparable to ascorbic acid (112.38 μg/mL). ABTS radical scavenging results further confirmed the superior antioxidant potential of EEPO (IC₅₀ 75.87 μg/mL) and EAEPO (IC₅₀ 123.73 μg/mL). Tyrosinase inhibition studies discovered that EAEPO and EEPO significantly inhibited mushroom tyrosinase activity in a concentration-dependent manner, comparable to standard inhibitors. The enhanced biological activities of EAEPO and EEPO correlate with their high phenolic, flavonoid, and tannin contents, as confirmed by quantitative estimations. The findings suggest that ethyl acetate and ethanol extracts of Pleurotus ostreatus are promising natural sources of antioxidant and tyrosinase inhibitory compounds, supporting their potential application in cosmeceutical and pharmaceutical formulations for skin depigmentation and oxidative stress management.
Keywords: N/A.
How to cite this article: Ohol A, Wagh M, Inamdar N. Antityrosinase and Antioxidant Activity of Various Extracts of Oyster Mushroom i.e. Pleurotus ostreatus. Int J Drug Deliv Technol. 2026;16(7s): 901-913; DOI: 10.25258/ijddt.16.7s.96
Source of support: Nil
Conflict of interest: None