1PG and Research Department of Microbiology, Cauvery College for Women (Autonomous), Affiliated to Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
2*Assistant Professor, Reproductive Endocrinology Lab, Department of Research, Saveetha College of Nursing, Saveetha Institute of Medical and Technical Sciences (SIMATS), Thandalam, Chennai, Tamil Nadu, India
Email: anbubot373@gmail.com; thirumurugana.scon@saveetha.com
Probiotics are live microorganisms that, when given in the right doses, can improve the health of the person who takes them. In this work, Lactobacillus species were isolated from the breast milk of a 26-year-old woman and assessed for their antibacterial efficacy against certain vaginal infections. The isolates underwent testing at varying concentrations (50 µL, 40 µL, and 30 µL). Motility tests, Gram staining, and routine biochemical assays were used to make a first guess about what Lactobacillus was. The agar disc diffusion method was used to test the isolated strain's ability to kill vaginal infections. Antibiotic susceptibility testing was also performed on the pathogenic isolates. The findings indicated that the Lactobacillus strain derived from breast milk shown considerable antibacterial efficacy against the examined vaginal pathogens, particularly demonstrating the most pronounced inhibitory effect on Klebsiella pneumoniae. We used Gas Chromatography–Mass Spectrometry (GC–MS) to do more research and find the bioactive components in the extract. The investigation showed that the active fraction had bioactive chemicals that could be new. The main goal of this study was to find out if Lactobacillus from breast milk may help prevent cervical and vaginal infections by testing its ability to kill bacteria and finding the bioactive substances that do so. These discoveries may expand existing research paradigms and facilitate the advancement of natural, human-derived medicinal molecules with reduced adverse effects relative to traditional chemical pharmaceuticals.
Keywords: Probiotic, Vaginal pathogens, Lactobacillus, LMW compounds, GC-MS, Antimicrobial compounds.
How to cite this article: Sandhiya T, Jeyabharathi S, Thirumurugan A. Exploration of Antimicrobial Drug Targets from Human Breast Milk Lactobacillus for the Control of Vaginal Infections. Int J Drug Deliv Technol. 2026;16(7s): 924-933; DOI: 10.25258/ijddt.16.7s.98
Source of support: None
Conflict of interest: None