Department of Botany, Dada Patil Rajale College, Adinathnagar, Tal-Pathardi, Dist-Ahmednagar-414505 (MS) India
E-mail: jnnehul@gmail.com
A strain of cyanobacterium Westiellopsis prolifica was isolated from soil samples collected from different locations of Pathardi tehsil of Ahmednagar district. Identification using morphological variation and taxonomic methods. Sterile cultures of Westiellopsis prolifica were obtained in laboratory. Use a different medium, BG-11 medium, for biomass production. Collect the biomass by filtration through two layers of muslin and dry using a hair dryer. Westiellopsis prolifica cultures were isolated by improving soil samples in BG-11 medium (Kaushik 1987). Sterile cultures were obtained by repeated liquid transfer of small particles followed by antibiotic treatment (streptomycin, chloramphenicol and penicillin (10 mg ml⁻¹)) (Kaushik, 1987). Medium and clean Unalgal culture. The extraction process requires solvents of different polarities such as (i) hexane (ii) chloroform (iii) methanol and (iv) distilled water. Biomass samples were weighed and added to each weight at a ratio of 1:10 (w/v) for the removal of metabolites. Three consecutive extract filtrations were carried out using Whatman paper and the subsequent filtrates were collected. Treatment of HeLa cells with methanol, chloroform, hexane and aqueous extracts caused dose-dependent cell death as evidenced by the decrease in cell viability assessed by MTT assay. Extracts obtained from Westiellopsis prolifica were tested and found to have antitumor activity at different rates. The most potent is the extract of Westiellopsis prolifica.
Keywords: Westiellopsis prolifica, Cyanobacterium, Antitumor activity, HeLa cells, MTT assay, BG-11 medium
How to cite this article: Nehul JN. Investigation of Antitumor Activity of Different Extracts of Cyanobacterium Westiellopsis Prolifica Janet. Int J Drug Deliv Technol. 2026;16(8s): 1071-1074; DOI: 10.25258/ijddt.16.8s.120
Source of support: Nil.
Conflict of interest: None