Department of Microbiology, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth (Deemed to Be University), Karad-415539, Maharashtra, India
Corresponding Author:
Dr. Harsha V. Patil
Associate Professor, Department of Microbiology, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth (Deemed to Be University), Karad-415539, Maharashtra, India
Email: harshavpatil@gmail.com
In rural Indian hospitals, limited surveillance, inadequate diagnostics, and antibiotic misuse hinder AMR control; this study evaluated Pseudomonas aeruginosa (P. aeruginosa) isolation, antimicrobial susceptibility, and AmpC β-lactamase prevalence. A total of 187 P. aeruginosa isolates obtained from 55 clinical samples were analysed during this study. The overall prevalence of AmpC β-lactamase production was 33.16%. Our study's AmpC prevalence remained within the upper range of Indian reports, higher than earlier low-prevalence reports with resistance burden. Male predominance was observed, with high AmpC prevalence in ICU and urine isolates, and the highest number of isolates from patients aged 41–60 years (38.75%). A high prevalence of AmpC β-lactamase–producing P. aeruginosa was detected, especially in ICU and urine isolates, with widespread resistance to both β-lactam and non-β-lactam antibiotics. Maximum resistance was noted against cefoxitin (100%), followed by piperacillin (52.5%), ciprofloxacin, ceftazidime, and meropenem (each 51.25%). The isolates showed the best response to piperacillin–tazobactam (61.25%), followed by imipenem and amikacin (56.25% each) and aztreonam (51.25%). In contrast, very low sensitivity was observed to ceftazidime (16.1%) and piperacillin–tazobactam (8.5%). AmpC producers were isolated from urine samples (25%) and ICU patients (32.26%). The cefoxitin–cloxacillin double-disk synergy test showed the highest detection rate (63.75%), followed by the disk approximation test (48.75%) and the boronic acid disk test (45%). These findings highlight the need for routine AmpC screening, stronger antimicrobial stewardship, and effective infection control in both tertiary and rural healthcare settings in India to limit the spread of multidrug-resistant P. aeruginosa.
Keywords: Pseudomonas aeruginosa, antibiogram, AmpC-β-lactamases, multidrug-resistant (MDR), antimicrobial resistance (AMR).
How to cite this article: Pachakar AA, Patil HV. Study of AmpC β-Lactamase Producing Pseudomonas Aeruginosa from Clinical Samples in Tertiary Care Centre. Int J Drug Deliv Technol. 2026;16(8s): 239-247; DOI: 10.25258/ijddt.16.8s.35
Source of support: None
Conflict of interest: None