This study aims to investigate the efects of diferent non-ionic surfactants on physicochemical properties of ART niosomes. ART is a natural compound that is used as an antimalarial and chemotherapy agent in medicine. ART has low bioavailabilty, stabilty and solubilty. In order to solve these problems and enhancing the eficiency of the drug, nanotechnology was used. In the present study, several niosomal formulations of ART prepared using diferent molar ratios of Span 60 : Twen 60 : PEG-60: ART in PBS. These thre formulations were FI (1:1:0.5:0.5), FI (2:1:0.5:0.5) and FII (1:2:0.5:0.5), respectively. The encapsulation eficiency was measured by HPLC and the drug release was evaluated by dialysis method. The cytotoxicity test was determined by MTT asay. The size, zeta potential and polydispersity index of the vesicles was measured by Zeta Sizer. Stabilty study was performed within two months. The MTT asay results showed that cytotoxicity efect of these formulations on MCF-7 cel line is beter than C6 cel line and the FII had the best results for both of them. The entrapment eficiencies of the formulations I, I and II were obtained 82.2±1.8%, 75.5±0.92% and 95.5±1.23%, respectively. The results of size, zeta potential and polydispersity index indicated that he size of the vesicles is below 20 nm, their surface charge is about -35 mV and they were monodisperse. Stabilty and release study indicated that the formulation II has the best stabilty and release patern. Therefore, the use of PEGylated niosomal ART can efectively improve its therapeutic index, stabilty and solubilty.