Title:

Formulation and Development of Bioactive Electrospun Nanofibers for Regenerative Medicine: Egg Yolk Oil and Rosehip Seed Oil   Novel Biomaterial-Based Approaches

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Author:

Akiladevi Durairaj¹*, Mohamed Shaik Fareed G.², Nirmal Kumar J.², A. Jerad Suresh³

¹*Professor & Head, Department of Pharmaceutics, School of Pharmaceutical sciences, Vels Institute of Science Technology and Advanced studies, Pallavaram, Chennai 600117.

² Research Scholar, Department of Pharmaceutics, School of Pharmaceutical Sciences, Vels Institute of Science Technology and Advanced Studies, Pallavaram, Chennai 600117.

³Professor and Principal, Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (Deemed to be University), Porur, Chennai-600116.

 Received: 20^th Jul, 2025; Revised: 9^th Sep 2025; Accepted: 15^th Nov, 2025; Available Online: 30^th Nov, 2025

 

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ABSTRACT

This study reports the formulation and evaluation of bioactive electrospun nanofibers incorporating egg yolk oil (EYO) and rosehip seed oil (RSO) within polycaprolactone–gelatin scaffolds for regenerative medicine. Optimized nanofibers displayed uniform morphology, high encapsulation efficiency (>85%), and sustained release profiles exceeding 90% within 48 hours. FTIR and DSC confirmed compatibility of oils with polymers, while SEM revealed bead-free fiber structures. Mechanical testing indicated sufficient tensile strength for scaffold stability. In vitro assays demonstrated excellent cytocompatibility, with nanofiber-treated groups showing >95% cell viability compared to controls. Anti-apoptotic studies confirmed enhanced Bcl-2 expression and reduced cleaved caspase-3, indicating suppression of apoptosis. ELISA further validated upregulation of antioxidant and regenerative markers. Compared to synthetic scaffolds, EYO-based nanofibers provided superior cell adhesion, metabolic activity, and faster release kinetics, whereas RSO-based scaffolds offered prolonged bioactivity. Collectively, these results suggest that natural oil-infused nanofibers can synergize with polymers to create bioactive, biocompatible scaffolds suitable for wound healing, bone regeneration, and broader regenerative applications. Future work should address long-term stability, large-scale production, and regulatory compliance to facilitate clinical translation…

Keywords: Electrospinning, Nanofibers, Egg yolk oil, Rosehip seed oil, Polycaprolactone, Gelatin, Tissue engineering.

How to cite this article: Durairaj A, Fareed GMS, Kumar NJ, Suresh AJ. Formulation and development of bioactive electrospun nanofibers for regenerative medicine: egg yolk oil and rosehip seed oil novel biomaterial-based approaches. Int J Drug Deliv Technol. 2025; 15(4):74–80.

Source of support: Nil.

Conflict of interest: None

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